Prior to assessing anti-inflammatory properties of cannabis extract, the ability of LPS to induce secretion of key cytokine-related factors was assessed in dermal fibroblasts. After 24 h of LPS stimulation, dermal fibroblast conditioned media contained substantially elevated levels of cytokine IL-6 and chemokine IL-8 & Figure 7). Our results show that accumulation of the indicated cytokines in conditioned media of dermal fibroblasts was significantly suppressed by cannabis extract treatment confirming potential anti-inflammatory property of the high CBD cannabis extract preparation that may be considered to target chronic inflammation in prostate cancer. Prostate cancer cells are in general highly resistant to common chemotherapeutic agents and the presence of CSCs is suggested to contribute to chemo resistance. The ability to form spheres in non-adherent, serum free conditions is a key property of stem cells.In this study, prostate cancer cell line LNCaP was able to form spheroids in non-adherent culture, suggesting the presence of cancer stem-like cells within these cell lines. Because prostaspheres are enriched with CSCs , the inhibitory effect of cannabis extract on prostasphere formation supports that high CBD and low THC cannabis extract may be a potent agent in targeting or eliminating prostate cancer stem-like cells in vitro .
Recently, cannabinoids have received considerable attention due to their diverse pharmacologic activities such as cell growth inhibition, anti-inflammatory effects, and tumor regression. Our results suggest that treatment of androgen-responsive human prostate carcinoma LNCaP cells resulted in a decrease in intracellular and secreted levels of PSA, with concomitant inhibition of androgen receptor, cell growth, and induction of apoptosis. The data also demonstrate that cannabis extract is capable of significantly suppressing the expression of specific pro-inflammatory cytokine/chemokine in human dermal fibroblast cells. The study explains usefulness of LPS-stimulated in vitro systems for the evaluation of the anti-inflammatory properties of plant extracts using this methodological approach . To conclude, the data presented in this paper provide further support for the concept that traditional medicines, such as cannabis, can be valuable additions to the modern therapeutic armamentarium, and non-habit-forming cannabinoid agonist which lack psychotropic activity may be used for the management of prostate cancer. Some studies have reported the application of marijuana in the treatment of HIV patients to improve their food intake and promote their appetite by increasing ghrelin and leptin hormones,and decreasing peptide tyrosine hormone responsible for appetite regulation.
In addition, the application of medicinal marijuana in management of coronavirus disease 2019 has been explored by a number of scientific studies.This disease had not found any medical cure by the time this review was done. Therefore in the search for a vaccination or cure, the application of Marijuana has been explored by scientists who have given preliminary findings showing that the plant may offer resistance to SARS-CoV-2 .The principle behind this is based on the fact that medicinal cannabis has been used to treat nausea and dementia, thus offering hopes that the angiotensin converting enzyme IIreceptor needed by the corona virus found in the lung tissues, oral and nasal mucus, and gastrointestinal tracts can be modulate dusing cannabis extracts and removed from the biological system in order to inhibit vulner ability against the virus and therefore reduce its infection risks .Furthermore, terpenes from cannabis have exhibited potent characteristics as antiviral agents with the ability to minimize the severity and impact of SARS virus by suppressing the protein responsible for RNA replication and thereby blocking the virus from penetrating the cells .
For instance, it has been emphasized that marijuana extracts can reduce or regulate serine protease TMPRSS2,mainly required by the SARS-Cov-2 in order to gain entry into the human host cells. Consequently, cannabis has been reported to be effective in the prevention and treating of COVID-19 even though there are limited scientific studies that have been performed to support or refute these assertions, especially the role played by cannabinoids towards viral therapy. Cannabis just like any other drug substance once taken into the body system it describes a systematic pathway from the time it gains entry into the body system up to the time it is excreted through series of biological activities that involve absorption, distribution, metabolism and excretion, all which are dependent on the drug bioavailability that determines the onset, duration and the intensity of effects manifested by the drug .